Trigonella foenum-graecum Seed Dry Extract

Trigonella foenum-graecum Seed Dry Extract

Final Authorized Version 1.0

Trigonella foenum-graecum Seed Dry Extract


 

DEFINITION

The article is prepared from the dried ripe seeds of Trigonella foenum-graecum L. (Family Fabaceae) by extraction with hydroalcoholic mixtures. The ratio of starting crude plant material to Dry Extract is between 5:1 and 4:1. It contains NLT 1.0% of 4-hydroxyisoleucine, calculated on the anhydrous basis.

 

POTENTIAL CONFOUNDING MATERIALS

None known

 

CONSTITUENTS OF INTEREST

Amino acids: 4-Hydroxyisoleucine, 4-hydroxyisoleucine lactone, arginine, histidine, and lysine

Steroidal saponins: Trigoneoside IIa, Ib; graecunins H, I, J, K, L, M, N; trigofoenosides A, D, F, G; protogracillin; protodioscin; and diosgenin

Alkaloids: Trigonelline, gentianine, and carpaine

Flavonoids: Apigenin, luteolin, orientin, quercetin, vitexin, and isovitexin

Carbohydrates: Mainly mucilage forming polysaccharides

 

IDENTIFICATION

• A. Thin-Layer Chromatography—Amino Acids Profile

Standard solution A: 0.5 mg/mL of USP 4-Hydroxyisoleucine RS in methanol

Standard solution B: 50 mg/mL of USP Trigonella foenum-graecum Seed Dry Extract RS in methanol. Sonicate for 10 min, centrifuge, and use the supernatant.

Sample solution: 50 mg/mL of Trigonella foenum-graecum Seed Dry Extract in methanol. Sonicate for 10 min, centrifuge, and use the supernatant.

[Note—Save the Sample solution for use in Identification B and the Specific Test­s—Presence of Trigonelline.]

Chromatographic system

(See Chromatography <621>, Thin-Layer Chromatography.)

Adsorbent: Chromatographic silica gel mixture with an average particle size of 5 µm (HPTLC plates)

Application volume: 2 µL, as 8-mm bands

Relative humidity: Condition the plate to a relative humidity of about 33% using a suitable device.

Temperature: 25°

Developing solvent system: n-Butanol, acetic acid, and water (7:2:1)

Developing distance: 6 cm

Derivatization reagent: Ninhydrin reagent created by combining 0.3 g of ninhydrin, 95 mL of isopropanol, and 5 mL of glacial acetic acid.

Analysis

Samples: Standard solution A, Standard solution B, and Sample solution

Apply the Samples as bands to a suitable HPTLC plate and dry in air. Develop the chromatograms in a saturated chamber, remove the plate from the chamber, and dry. Treat with Derivatization reagent, heat for 2 min at 100°–105°, and examine under visible light and UV light at 366 nm.

System suitability: Under visible light, the chromatogram of Standard solution B exhibits, in the lower half, five bands: the most intense band at an RF corresponding to the 4-hydroxyisoleucine band of Standard solution A, one minor band just below the 4-hydroxyisoleucine band, and three minor bands above the 4-hydroxyisoleucine band.

Under UV light at 366 nm, the chromatogram of Standard solution B exhibits, in the lower half, four bands: the most intense band as a very dark band at an RFcorresponding to the 4-hydroxyisoleucine band of Standard solution A, one minor dark band just below the 4-hyroxyisoleucine band, and two dark bands above the 4-hydroxyisoleucine band. The chromatogram of Standard solution B exhibits, in the upper half, three bright yellow bands, the one with the lowest RF appears diffuse.

Acceptance criteria: Under visible light, the chromatogram of the Sample solution exhibits, in the lower half, the most intense band at an RF corresponding to the 4-hydroxyisoleucine band of Standard solution A, and four additional brown bands corresponding to similar bands of Standard solution B: a minor band at an RF below that of the 4-hydroxyisoleucine, and three less intense bands above the 4-hydroxyisoleucine band.

Under UV light at 366 nm, the chromatogram of the Sample solution exhibits, in the lower half, the most intense band at an RF corresponding to the 4-hydroxyisoleucine band of Standard solution A, and three additional bands corresponding to similar bands of Standard solution B: a minor dark band at an RF below that of the 4-hydroxyisoleucine, and two less intense bands above the 4-hydroxyisoleucine band. The chromatogram of the Sample solution exhibits, in the upper half, three bright yellow bands corresponding to similar bands of Standard solution B.

• B. Thin-Layer Chromatography—Steroidal Saponins Profile

Standard solution: 50 mg/mL of USP Trigonella foenum-graecum Seed Dry Extract RS in methanol. Sonicate for 10 min, centrifuge, and use the supernatant.

Sample solution: Use the Sample solution prepared in Identification A.

Chromatographic system

(See Chromatography <621>, Thin-Layer Chromatography.)

Adsorbent: Chromatographic silica gel mixture with an average particle size of 5 µm (HPTLC plates)

Application volume: 2 µL, as 8-mm bands

Relative humidity: Condition the plate to a relative humidity of about 33% using a suitable device. [Note—Suitable device includes MgCl2.]

Temperature: 25°

Developing solvent system: Dichloromethane, methanol, and water (18:8:1)

Developing distance: 6 cm

Derivatization reagent: Anisaldehyde reagent created by combining 85 mL of ice-cooled methanol mixed with 10 mL of glacial acetic acid, 5 mL of sulfuric acid, and 0.5 mL of p-anisaldehyde.

Analysis

Samples: Standard solution and Sample solution

Apply the Samples as bands to a suitable HPTLC plate and dry in air. Develop the chromatograms in a saturated chamber, remove the plate from the chamber, and dry. Treat with Derivatization reagent, heat for 3 min at 100°, and examine under visible light and UV light at 366 nm.

System suitability: Under visible light, the chromatogram of the Standard solution exhibits, in the lower half, five intense bands in the following order of increasing RF: a brown band close to the origin, a brown band due to fructose, a brown band due to protogracillin, a brown band due to protodioscin, and a violet band. The chromatogram of the Standard solution exhibits, in the upper half, two violet bands and a third violet band close to the solvent front.      

Under UV light at 366 nm, the chromatogram of the Standard solution exhibits, in the lower third, three blue fluorescent bands interspersed with two brown bands due to saponins; a broad blue fluorescent band at about the middle of the run; and a pink band in between.     

Acceptance criteria: Under visible light, the chromatogram of the Sample solution exhibits, in the lower half, three intense brown bands and a violet band corresponding in RF to similar bands of the Standard solution, and three violet bands in the upper half corresponding in R to similar bands of the Standard solution.

Under UV light at 366 nm, the chromatogram of the Sample solution exhibits, in the lower third, three blue fluorescent bands interspersed with two brown bands corresponding in R to similar bands of the Standard solution; a pink band about in the middle of the chromatogram, and a broad blue fluorescent band below it corresponding in R to similar bands of the Standard solution

 

ASSAY

• Content of 4-Hydroxyisoleucine

Solution A: 0.1% Phosphoric acid in water (v/v)

Solution B: Acetonitrile

Mobile phase: See Table 1.

 

Table 1

Time
(min)

Solution A
(%)

Solution B
(%)

0

80

20

20

40

60

 

Reagent: Acetonitrile, water, and triethylamine (40:12:8)

Diluent: Methanol and water (1:1) 

Standard solution: Transfer about 2.0 mg of USP 4-Hydroxyisoleucine RS, accurately weighed, to a 100-mL volumetric flask, dissolve in 10 mL of Reagent, add 500 µL of phenyl isothiocyanate, and shake for 5 min. Add 60 mL of methanol, complete to volume with water, and mix.

Sample stock solution: Transfer about 0.2 g of Trigonella foenum-graecum Seed Dry Extract, accurately weighed, to a 10-mL volumetric flask, add 8 mL of Diluent, sonicate to dissolve, cool, complete to volume with Diluent, mix, and filter.

Sample solution: Transfer 5.0 mL of Sample stock solution to a 100-mL volumetric flask, add 10 mL of Reagent and 500 µL of phenyl isothiocyanate, and shake for 5 min.  Add 60 mL of methanol, complete to volume with water, and mix. Before injection, pass through a membrane filter of 0.45-µm or finer pore size, discarding the first few mL of the filtrate.

Chromatographic system

(See Chromatography <621>, System Suitability.)

Mode: LC

Detector: UV 254 nm

Column: 4.6-mm × 15-cm; 5-µm packing L1 (Similar to Zorbax Eclipse XD-C18, Luna C18(2) and Cosmosil C18-MS-II)

Flow rate: 1.5 mL/min

Injection volume: 20 µL

System suitability

Sample: Standard solution

Suitability requirements

Tailing factor: NMT 2.0 for the 4-hydroxyisoleucine peak

Relative standard deviation: NMT 2.0% determined from the 4-hydroxyisoleucine peak in repeated injections

Analysis

Samples: Standard solution and Sample solution

Using the chromatogram of the Standard solution, identify the retention time of the peak corresponding to 4-hydroxyisoleucine in the Sample solution chromatogram.

Calculate the percentage of 4-hydroxyisoleucine in the portion of Trigonella foenum-graecum Seed Dry Extract taken:

 

Result = (rU/rS) × (CS/CU) × d × 100

 

rU    = peak area of 4-hydroxyisoleucine from the Sample solution

rS    = peak area of 4-hydroxyisoleucine from the Standard solution

C  = concentration of 4-hydroxyisoleucine in the Standard solution (mg/mL)

CU   = concentration of Trigonella foenum-graecum Seed Dry Extract in the Sample stock solution (mg/mL)

d      = dilution factor to prepare the Sample solution from the Sample stock solution 

Acceptance criteria: NLT 1.0% on the anhydrous basis

 

CONTAMINANTS

Elemental Impurities—Procedures <233>

Acceptance criteria

Arsenic: NMT 2.0 µg/g

Cadmium: NMT 0.5 µg/g

Lead: NMT 5.0 µg/g

Mercury: NMT 0.2 µg/g

Articles of Botanical Origin, General Method for Pesticide Residues Analysis <561>: Meets the requirements

Microbial Enumeration Tests <61>: The total aerobic bacterial count does not exceed 104 cfu/g and the total combined molds and yeasts count does not exceed 102 cfu/g.

Tests for Specified Microorganisms <62>: Meets the requirements of the tests for the absence of Salmonella species and Escherichia coli

 

SPECIFIC TESTS

• Presence of Trigonelline

Standard solution A: 1.5 mg/mL of USP Trigonelline RS in methanol

Standard solution B: 50 mg/mL of USP Trigonella foenum-graecum Seed Dry Extract RS in methanol. Sonicate for 10 min, centrifuge, and use the supernatant.

Sample solution: Use the Sample solution prepared in Identification A.

Chromatographic system

(See Chromatography <621>, Thin-Layer Chromatography.)

Adsorbent: Chromatographic silica gel mixture with an average particle size of 5 µm (HPTLC plates)

Application volume: 5 µL, as 8-mm bands

Relative humidity: Condition the plate to a relative humidity of about 33% using a suitable device.

Temperature: 25°

Developing solvent system: Isopropyl alcohol, methanol, and water (4:1:4)

Developing distance: 6 cm

Analysis

Samples: Standard solution A, Standard solution B, and Sample solution

Apply the Samples as bands to a suitable HPTLC plate and dry in air. Develop the chromatograms in a saturated chamber, remove the plate from the chamber, dry, and examine under UV light at 254 nm.

System suitability: Under UV light, the chromatogram of Standard solution B exhibits, in the lower half, a quenching band at an RF similar to the trigonelline band of Standard solution A. A strong quenching band is near the solvent front of the chromatogram.

Acceptance criteria: Under UV light, the chromatogram of Standard solution B exhibits, in the lower half, a quenching band corresponding in Rto the trigonelline band of Standard solution A.

Water Determination, Method Ia <921>: NMT 9.0%

 

ADDITIONAL REQUIREMENTS

Packaging and Storage: Preserve in well-closed containers, protected from light and moisture, and store at room temperature.

Labeling: The label states the Latin binomial and, following the official name, the part(s) of the plant from which the article was derived. It meets other labeling requirements under Botanical Extracts <565>.

USP Reference Standards <11>

USP 4-Hydroxyisoleucine RS

USP Trigonella foenum-graecum Seed Dry Extract RS

USP Trigonelline RS

Other Versions

Proposed For Comment Version 0.2