Vitex negundo Leaf Dry Extract

DEFINITION

Vitex negundo Leaf Dry Extract consists of the dried leaves of Vitex negundo L. (Fam. Lamiaceae), commonly known as Chinese Chaste Tree or Five-Leaf Chaste Tree, by extraction with hydroalcoholic mixture. It contains NLT 2.5% of iridoid glycosides, calculated as the sum of agnuside and negundoside on the dried basis.

POTENTIAL CONFOUNDING MATERIALS

Vitex agnus-castus L.

Vitex rotundifolia L.f

Vitex trifolia L.

CONSTITUENTS OF INTEREST

Iridoid glycosides: Negundoside, nishindaside, and agnuside

IDENTIFICATION

• A. Thin-Layer Chromatography

Standard solution A: 3 mg/mL each of USP Negundoside RS...