Trigonella foenum-graecum Seed 4-Hydroxyisoleucine Powdered Extract

Trigonella foenum-graecum Seed 4-Hydroxyisoleucine Powdered Extract

DEFINITION

The article is a fraction of an extract of the dried ripe seeds of Trigonella foenum-graecum L. (Family Fabaceae). The extract is prepared using hydroalcoholic mixtures then further enriched in 4-hydroxyisoleucine using suitable means. It contains NLT 90.0% and NMT 110.0% of the labeled amount of 4-hydroxyisoleucine, calculated on the anhydrous basis.

POTENTIAL CONFOUNDING MATERIALS

None known

CONSTITUENTS OF INTEREST

Amino acids: 4-Hydroxyisoleucine, 4-hydroxyisoleucine lactone, arginine, histidine, and lysine

IDENTIFICATION

• Thin-Layer Chromatography—Amino Acids Profile

Standard solution A: 0.5 mg/mL of USP 4-Hydroxyisoleucine RS in...